• Duvic B., Jouan V., Girard P.A., Pagès S., Abi Khattar Z., Volkoff N.A., Givaudan A., Destoumieux-Garzón D., and Escoubas J.M. (2012) Cecropins as a marker of Spodoptera frugiperda immunosuppression during entomopathogenic bacterial challenge. J. Insect. Physiol. DOI : 10.1016/j.jinsphys.2012.04.001
• Marie B., Joubert C., Belliard C., Tayale A., Zanella-Cléon I., Marin F., Gueguen Y., and Montagnani C. (2012). Characterization of MRNP34, a novel methionine-rich nacre protein from the pearl oysters. Amino acids  42:2009–2017.
• Rosa R.D., de Lorgeril J., Tailliez P., Bruno R., Piquemal D., and Bachère E. A hemocyte gene expression signature correlated with the predictive capacity of oysters to survive Vibrio infections. BMC genomics (accepted).
• Schmitt P, Duperthuy M, Montagnani C, Bachère E and Destoumieux-Garzón D. Immune responses in the Pacific oyster Crassostrea gigas. An overview with focus on summer mortalities. In Oysters: Physiology, Ecological Distribution and Mortality. Editor Jian Qin. Edition NovaPublishers. (in press)
• Schmitt P., de Lorgeril J., Gueguen Y., Destoumieux-Garzón D., and Bachère E. (2012). Expression, tissue localization and synergy of antimicrobial peptides and proteins in the immune response of the oyster Crassostrea gigas. Dev. Comp. Immunol., DOI : 10.1016/j.dci2012.01.004.
• Schmitt P., Rosa R.D., Duperthuy M., de Lorgeril J, Bachère E., and Destoumieux-Garzón D. The antimicrobial defense of the Pacific oyster, Crassostrea gigas. How diversity may compensate for scarcity in the regulation of resident/pathogenic microflora. Frontiers in Antimicrobials, Resistance and Chemotherapy (in press)

• Duperthuy M, Schmitt P, Garzón E, Caro A, D Rosa R, Le Roux F, Lautrédou-Audouy N, Got P, Romestand B, de Lorgeril J, Kieffer-Jaquinod S, Bachère E, and Destoumieux-Garzón D (2011) Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus. Proc Natl Acad Sci U S A 108(7):2993-8.
• de Lorgeril J, Zenagui R, Rosa R, Piquemal D, and Bachère E. Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis. PlosOne 6(8):e23142.
• Montagnani C, Marie B, Marin F, Belliard C, Riquet F, Tayalé A, Zanella-Cléon  I, Fleury E, Gueguen Y, Piquemal D, and  Cochennec-Laureau N. Pmarg-Pearlin is a matrix protein involved in nacre framework formation in the pearl oyster Pinctada margaritifera. ChemBioChem 12(13): 2033-43
• Nguikwie SK, Nyegue MA, Ndoye FCB, Ngono Ngane A, Romestand B, Casabianca H, Amvam Zollo PH and Menut C. Essential oils of the Aframomum species collected in Cameroon as potential source of (E)- Nerolidol : Chemical and antibacterial activities. Food Chemistry (in press).
• Oukacine F, Garrelly L, Romestand B, Goodall DM, Zou T, Cottet H. Focusing and Mobilization of Bacteria in Capillary Electrophoresis. Anal Chem 83(5) :1571-1578.
• Oukacine F, Joselito PQ, Garrelly L, Romestand B, Zou T, and Cottet H. Simultaneous Electrokinetic and Hydrodynamic Injection for high sensitivity bacteria analysis in Capillary Electrophoresis. Anal Chem 83: 499-4954
• Perazzolo LM, Bachère E, Rosa RD, Goncalves P, Andreatta ER, Daffre S, and Barracco MA. (2011) Alpha2-macroglobulin from an Atlantic shrimp: Biochemical characterization, sub-cellular localization and gene expression upon fungal challenge. Fish Shellfish Immunol.e2011/09/06
• Rosa, RD, Santini A, Fievet J, Bulet P, Destoumieux-Garzon D, and Bachère E (2011) Big Defensins a diverse family of antimicrobial peptides that follows different patterns of expression in hemocytes of the oyster Crassostrea gigas. PLoS One, 2011. 6(9): p. e25594
• Thomas Y, Belliard C, Garen P, Gueguen Y, and Montagnani C. (2011). Development of in situ hybridisation using 16S rRNA gene to monitor black-lip pearl oyster, Pinctada margaritifera, larvae in plankton samples. Aquat Living Resour. 24: 27-34.
• Vassiliadis G., Destoumieux-Garzόn D, and Peduzzi J. (2011) Class II Microcins. In Prokaryotic antimicrobial peptides: from genes to applications. Editors: D. Drider & S. Rebuffat, Ed. Springer. pp309-332
• Vidal-Dupiol J, Ladrière O, Destoumieux-Garzόn D, Sautière PE, Meistertzheim AL, Tambutté E, Tambutté S, Duval D, Fouré L, Adjeroud M, and Mitta G. Innate immune responses of a scleractinian coral to vibriosis. J Biol Chem. 286, 22688-22698.

• Cochennec-Laureau N, Montagnani C, Saulnier D, Fougerouse A, Levy P, and Lo C. (2010) A histological examination of grafting success in pearl oyster Pinctada margaritifera in French Polynesia. Aquat Living Resour. 23, 131-140.
• David R, Tréguier C, Montagnani C, Belliard C, Levy P, Nédélec G, Joufoques V, Remoissenet G, Gueguen Y, Cochennec-Laureau N. (2010) Molecular detection of betanodavirus from the farmed fish, Platax orbicularis (Forsskal) (Ephippidae), in French Polynesia. J Fish Dis. 33(5):451-4.
• Duperthuy M, Binesse J, Le Roux F, Romestand B, Caro A,  Got P,  Givaudan A, Mazel D, Bachère E, and Destoumieux-Garzón D. (2010) The major outer-membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas. Env Microbiol 12(4):951-63
• Jackson DJ, McDougall C, Woodcroft B, Moase P, Rose RA, Kube M, Reinhardt R, Rokhsar DS, Montagnani C, Joubert C, Piquemal D, Degnan BM. (2010) Parallel evolution of nacre building gene sets in molluscs. Mol Biol Evol. 27(3):591-608.
• Joubert C, Piquemal D, Marie B, Manchon L, Pierrat F, Zanella-Cléon I, Cochennec-Laureau N, Gueguen Y, Montagnani C. (2010) Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization. BMC Genomics. 11:613.
• Mazelet L, Dietrich J, and Rolland JL (2010). New RT-qPCR assay for viral nervous necrosis virus detection in sea bass, Dicentrarchus labrax (L.): Application and limits for hatcheries sanitary control. Fish Shellfish Immunol (in press).
• Rolland JL, Abdelouahab M, Dupont J, Lefevre, F, Bachère E, and B. Romestand. (2010) Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris. Mol Immunol  47(6):1269-77
• Romestand B, Rolland JL, Commeyras A, Coussot G, Desvignes I, Pascal R, Vandenabeele-Trambouze O. (2010) Dendrigraft poly-L-lysine: a non-immunogenic synthetic carrier for antibody production. Biomacromolecules. 11(5):1169-73.
• Schmitt P, Gueguen Y, Desmarais E, Bachère E, de Lorgeril J. (2010) Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas. BMC Evol Biol 25:10:23.
• Schmitt P, Wilmes M, Pugniere M, Aumelas A, Bachère E, Sahl HG, Schneider T, Destoumieux-Garzón D. (2010) Insight into invertebrate defensin mechanism of action: oyster defensins inhibit peptidoglycan biosynthesis by binding to lipid II. J Biol Chem. 285(38):29208-16
• Vassiliadis G, Destoumieux-Garzón D, Lombard C, Rebuffat S, Peduzzi J. (2010) Isolation and Characterization of two Members of the Siderophore-microcin Family, Microcins M and H47. Antimicrob Agents Chemother. 54(1):288-97

• Abi Khattar Z., Rejasse A, Destoumieux-Garzón D., Escoubas J. M., Sanchis V., Lereclus D., Givaudan A., Kallassy M., Nielsen-Leroux C., Gaudriault S. (2009) The dlt Operon of Bacillus cereus is Required for Resistance to Cationic Antimicrobial Peptides and Virulence in Insects. J. Bacteriol. 191(22):7063-73.
• Destoumieux-Garzón D., Brehelin M., Bulet P., Boublik Y., Girard P.A., Baghdiguian S., Zumbihl R., Escoubas J.M. (2009) Spodoptera frugiperda X-Tox Protein, an Immune Related Defensin Rosary, Has Lost the Function of Ancestral Defensins. PLOSone 4(8):e6795
• Fleury E, Huvet A, Lelong C, de Lorgeril J, Boulo V, Gueguen Y, Bachère E, Tanguy A, Moraga D, Fabioux C, Lindeque P, Shaw J, Reinhardt R, Prunet P, Davey G, Lapègue S, Sauvage C, Corporeau C, Moal J, Gavory F, Wincker P, Moreews F, Klopp C, Mathieu M, Boudry P, Favrel P. (2009) Generation and analysis of a 29,745 unique Expressed Sequence Tags from the Pacific oyster (Crassostrea gigas) assembled into a publicly accessible database: the GigasDatabase. BMC Genomics. 29;10:341.
• Gueguen Y., Romestand B., Fievet J., Schmitt P., Destoumieux-Garzón D., Vandenbulcke F., Bulet P., Bachère E. (2009). Oyster hemocytes express a proline rich peptide displaying synergistic antimicrobial activity with a defensin. Molecular Immunology 46:516-522
• Randrianarivelo R, Sarter S, Odoux E, Brat P, Lebrun M, Romestand B, Menu C, Andrianoelisoa HS, Raherimandimby M., Danthu P. (2009) Composition and antimicrobial activity of essential oils of Cinnamosma fragans. Food Chemistry, 114: 680-684.
• Yang Y., Boze H., Chemardin P., Padilla A., Moulin G., Tassanakajon A., Pugnière M., Roquet F., Destoumieux-Garzón D., Gueguen Y., Bachère E., Aumelas A. (2009). NMR structure of rALF-Pm3, an anti-lipopolysaccharide factor from shrimp. Model of the possible lipid A binding site. Biopolymers 91 : 207-220.

• De Lorgeril J., Gueguen Y., Goarant C., Goyard E., Mugnier C., Fievet J., Piquemal D., Bachère E. (2008). A relationship between antimicrobial peptide gene expression and capacity of a selected shrimp line to survive a Vibrio infection. Molecular Immunology 45(12): 3438-45.
• Giffard-Mena I., Lorin-Nebel C., Charmantier G., Castille R., Boulo V., (2008). Adaptation of the sea-bass (Dicentrarchus labrax) to fresh water: role of Aquaporins and Na+/K+-ATPases. Comparative Biochemistry and Physiology part- A 150: 332-338.
• Guinand B., Rolland JL., Bonhomme F (2008). Genetic structure of the common sole (Solea solea) in the Bay of Biscay: nurseries as units of selection? Estuarine Coastal and Shelf Science. 78 : 316-326
• Löfgren S.E., Miletti L.C., Steindel M., Bachère E., Barracco M.A. (2008). Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals. Experimental Parasitology 118(2):197-202.
• Montagnani C, Labreuche Y, Escoubas JM. (2008) Cg-IkappaB, a new member of the IkappaB protein family characterized in the pacific oyster Crassostrea gigas. Developmental & Comparative Immunology 32(3):182-90.
  
• Nebel C., Romestand B., Negre-Sadargues G., Aujoulat F., Grousset E., Bacal J., Bonhomme F., Charmantier G. (2008) Differential freshwater adaptation in juvenile sea-bass Dicentrarchus labrax: histophysiological basis at the gill and urinary system levels Journal of Experimental Biology. 208: 3859-3871.
• Roberts S., Gueguen Y., de Lorgeril J., Goetz F. (2008). Rapid accumulation of an interleukin 17 homolog transcript in Crassostrea gigas hemocytes following bacterial exposure. Developmental & Comparative Immunology  32(9):1099-104.
• Somboonwiwat K., Bachère E., Rimphanitchayakit V., Tassanakajon A. (2008). Localization of anti-lipopolysaccharide factor (ALFPm3) in tissues of the black tiger shrimp, Penaeus monodon, and characterization of its binding properties. Developmental & Comparative Immunology 32(10):1170-6.
• Tanguy A., Bierne N., Saavedra C., Pina B., Bachère E., Kube M., Bazin E., Bonhomme F., Boudry P., Boulo V., Boutet I., Cancela L., Dossat C., Favrel P., Huvet A., Jarque S., Jollivet D., Klages S., Lapègue S., Leite R., Moal J., Moraga D., Reinhardt R., Samain J.F., Zouros E., Canario A. (2008). Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution. Gene 408: 27-36.

• Boutet I., Lorin-Nebel C., de Lorgeril J. & Guinand B. (2007) Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions. Comp. Biochem. Physiol., D.  2(1): 74-83
• Carriel-Gomes M.C., Kratz J.M., Barracco M.A., Bachère E., Monte Barardi C.R., Oliveira Simões C.M. (2007). In vitro antiviral activity of antimicrobial peptides against herpes simplex virus 1, adenovirus, and rotavirus. Mem. Inst. Oswaldo Cruz, 102, 469-472.
• Duquesne S., Destoumieux-Garzón D., Peduzzi J., and Rebuffat S. (2007) Microcins, gene-encoded antibacterial peptides from enterobacteria. Nat. Prod. Rep. 24, 708-734
• Duquesne S., Destoumieux-Garzón D., Zirah S., Goulard C., Peduzzi J., and Rebuffat S. (2007) Two Enzymes Catalyze the Maturation of a Lasso Peptide in Escherichia coli. Chem. Biol. 14, 793-803
• Gonzalez M., Gueguen Y., Desserre G., de Lorgeril J., Romestand B., Bachère E. (2007). Molecular characterization of two isoforms of defensin from hemocytes of the oyster Crassostrea gigas. Dev.  Comp. Immunol. 31, 332-339.
• Gonzalez M., Gueguen Y., Destoumieux-Garzón D., Romestand B., Fievet J., Pugnière M., Roquet F., Escoubas J.M., Vandenbulcke F., Levy O., Sauné L., Bulet P., Bachère E. (2007) Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI. Proc Natl Acad Sci U S A. 104(45):17759-64.
• Jenny M.J., Chapman R.W., Mancia A., Chen Y.A., McKillen D.J., Trent H., Lang P., Escoubas J.M., Bachère E., Boulo V., John Liu Z., Gross P., Cunningham C., Cupit P.M., Tanguy A., Guo X., Moraga D., Boutet I., Huvet A., De Guise S., Almeida J.S. and Warr G. (2007). A cDNA microarray for Crassostrea virginica and C. gigas. Mar. Biotechnol., en ligne
• Pernice M., Destoumieux-Garzón D., Peduzzi J., Rebuffat S., and Boucher-Rodoni R. (2007) Identification of a Vibrio strain producing antimicrobial agents in the excretory organs of Nautilus pompilius (Cephalopoda, Nautiloidea). Rev. Fish Biol. Fisher. 17, 197-205
• Samain JF., L. Dégremont, P. Soletchnik, J. Haure, E. Bédier, M. Ropert, J.Moal, A. Huvet, H. Bacca, A. Van Wormhoudt, M. Delaporte, K. Costil, S. Pouvreau, C. Lambert, V. Boulo, P. Soudant, J.L. Nicolas, F. Le Roux, T. Renault, B. Gagnaire , F.Geret, I. Boutet, T. Burgeot, P. Boudry (2007) Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection processes. Aquaculture sous presse
• Tine M., de Lorgeril J., Panfili J., Diop K., Bonhomme F., and Durand J.-D. (2007) Growth hormone and Prolactin-1 gene transcription in natural populations of the black-chinned tilapia Sarotherodon melanotheron acclimatised to different salinities. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 147, 541-549.
• Vassiliadis G., Peduzzi J., Zirah S., Thomas X., Rebuffat S, and Destoumieux-Garzón D. (2007) Insight into siderophore-peptide biosynthesis: Enterobactin is a precursor for microcin E492 post-translational modification Antimicrob. Agents Chemother. en ligne

Location

The RIME team is situated at the first floor of the University of Montpellier 2 building number 24.

You can access to the lab via the main entrance of the building (central stairs).

Pictures and facilities' plan

Mohamed Reda Zenagui. "survival capacities of the Pacific oyster Crassostrea gigas following bacterial infcetion"

Defense: 13th july 2011 at Montpellier 2 University 

Rafael Da Rosa "molecular mechanism and genetic bases of survival capacities of the Pacific oyster to vibrioses: a transcriptomic exploring"

Defense: 17th november 2011 at Montpellier 2 University

Job offers

Nous proposons un contrat d’assistant ingénieur de 14 mois dans le cadre du projet ANR Vibriogène. Nous recherchons une personne expérimentée en bactériologie, biologie cellulaire et biologie moléculaire. Cette personne sera amenée à construire des mutants bactériens par échange allélique, effectuer des infections expérimentales in vivo (huîtres) et in vitro (culture primaires de cellules d’huîtres). Une expérience de la microscopie à épifluorescence, de  la microscopie confocale et de la cytométrie en flux serait un plus.

Le travail sera effectué dans l’équipe « Réponse Immunitaire des Macro-organismes marins et Environnement » de l’UMR 5119 « Ecologie des systèmes marins côtiers à Montpellier » à Montpellier. Le poste est à pourvoir pour Octobre 2012.

Contact. Delphine Destoumieux-Garzón.
Tel. 04 67 14 46 25
Email. This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

• Address
  

• Fax. 04 67 14 46 22